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KMID : 0617819930030010023
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Journal of Wonkwang Oriental Medicine
1993 Volume.3 No. 1 p.23 ~ p.32
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Study on the Cytotoxicity of Lonicerae Flos Extracts
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Abstract
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In order to investigate the cytotoxicity of Lonicerae Flos extract, their extracts were dissolved in distilled water and methanol.
All material diluted from 10^-3to 10^-7(㎎/㎖) with distilled water putted in each well of 24 multidish cultured rat fibroblast for 48hours. The cell number was calculated at 2 days and cell shape takes photographed by inverted microscope at same day.
The results were as follows:
1. The rate of cell multiplication was the lowest in water extract from 10^-3 to 10^-7(㎎/㎖) concentration and the highest in ether and hexane extract at 10^-7(㎎/㎖) concentration.
2. Index of cytotoxicity was the highest with 3 score in water extract at 10^-3(㎎/㎖) concentration and the lowest with 1 score in ether, ethyl acetate or hexane extract at same concentration.
3. The degeneration of cell shape and number was the severest in water extract of 10&-4(㎎/㎖) concentration, but ether and hexane extracts in same concentration were weaker than water extract.
These results indicate that the cytotoxicity of extracts by ether and hexane from same Lonicerae Flos was weaker then water extract.
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